Electron microscopy of SARS-CoV particles - Dataset 04

The dataset contains transmission electron microscopy image stacks (tomograms) of ultrathin sections through extracellular SARS-CoV particles in Vero cell cultures. The dataset contains 12 image stacks of slightly variable pixel dimensions, which were recorded at either 1.17 or 0.96 nm pixel size (12 bit). Image stacks were size calibrated and stored in 16 bit TIF format. Visualization can be done using ImageJ or Fiji. Each image stack in TIF format is supplemented by a file containing the corresponding raw image tilt series (MRC format; plus meta data files) generated by the tomography acquisition software and by a file with the aligned tiltseries. A PDF document describes the methods used for generation of the image files. The dataset was generated as dataset 04 for a comparative morphometric analysis of SARS-CoV and SARS-CoV-2. Further datasets which were used for the analysis are available in this repository (see dataset description document).

Related publication: Laue M, Kauter A, Hoffmann T, Möller L, Michel J, Nitsche A. Morphometry of SARS-CoV and SARS-CoV-2 particles in ultrathin plastic sections of infected Vero cell cultures. Sci Rep. 2021 Feb 10;11(1):3515. doi: 10.1038/s41598-021-82852-7. PMID: 33568700; PMCID: PMC7876034.