Effects of CB2 Receptor Agonist JWH133 on Aβ42-Induced Hippocampal Neurons

Objective To observe the effects of Cannabinoid type 2 receptor (CB2R) on Aβ-induced hippocampal neuron. Methods Hippocampal neurons were pre-treated with 10 μM JWH133 and then treated 100 nM Aβ42 oligomer for 7 day, the mRNA of Bcl-2 and Bax was measured by qRT-PCR, and the mitochondrial membrane potential and the production of ROS were measured by Flow Cytometry. Result Aβ42 oligomer significantly decreased the ratio of Bcl-2/Bax, and mitochondrial membrane potential, and increased the production of ROS. However, the pre-treatment JWH133 inhibited Aβ42-induced the ratio of Bcl-2/Bax decrease, and increased the mitochondrial membrane potential, decreased the production of ROS. Conclusion Activation of CB2R could inhibit the effects of Aβ42 on hippocampal neurons.

Read Complete Article at ijSciences: V92020062353 AND DOI: http://dx.doi.org/10.18483/ijSci.2353