Published August 5, 2020
| Version v1
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HIV-1 promoter is gradually silenced when integrated into BACH2 in Jurkat T-cells
Creators
- 1. Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich
- 2. Institute for Neuropathology, University Hospital Zurich
- 3. Institute for Transfusion Medicine and Gene Therapy, Medical Center, University of Freiburg
Description
Data from the study "HIV-1 promoter is gradually silenced when integrated into BACH2 in Jurkat T-cells". We developed a novel model using CRISPR/Cas9-mediated, targeted integration of our HIV-1-based, dual-fluorophore vector LTatCL[M], with which we can distinguish between latently and actively infected cells and specifically target any gene of interest. Using the HIV-1 integration hotspot BACH2 as example, we could show that HIV-1 integration into BACH2, but not into the safe-harbor locus AAVS1, leads to HIV-1 latency, which was reversible upon re-activation.
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raw_data_HIV-1 promoter is gradually silenced when integrated into BACH2 in Jurkat T-cells .zip
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(286.9 MB)
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