Preprint Open Access

The RNA Atlas, a single nucleotide resolution map of the human transcriptome

Lorenzi, Lucia; Chiu, Hua-Sheng; Cobos, Francisco Avila; Gross, Stephen; Volders, Pieter-Jan; Cannoodt, Robrecht; Nuytens, Justine; Vanderheyden, Katrien; Anckaert, Jasper; Lefever, Steve; Goovaerts, Tine; Birkballe Hansen, Thomas; Kuersten, Scott; Nijs, Nele; Taghon, Tom; Vermaelen, Karim; Bracke, Ken R.; Saeys, Yvan; De Meyer, Tim; Deshpande, Nandan; Anande, Govardhan; Chen, Ting-Wen; Wilkins, Marc R.; Unnikrishnan, Ashwin; De Preter, Katleen; Kjems, Jørgen; Koster, Jan; Schroth, Gary P.; Vandesompele, Jo; Sumazin, Pavel; Mestdagh, Pieter

The human transcriptome consists of various RNA biotypes including multiple types of noncoding RNAs (ncRNAs). Current ncRNA compendia remain incomplete partially because they are almost exclusively derived from the interrogation of small- and polyadenylated RNAs.Here, we present a more comprehensive atlas of the human transcriptome that is derived from matching polyA-, total-, and small-RNA profiles of a heterogeneous collection of nearly 300 human tissues and cell lines. We report on thousands of novel RNA species across all major RNA biotypes, including a hitherto poorly-cataloged class of non-polyadenylated single-exon long non-coding RNAs. In addition, we exploit intron abundance estimates from total RNA-sequencing to test and verify functional regulation by novel non-coding RNAs. Our study represents a substantial expansion of the current catalogue of human ncRNAs and their regulatory interactions. All data, analyses, and results are available in the R2 web portal and serve as a basis to further explore RNA biology and function.

Files (8.7 MB)
Name Size
807529v1.full.pdf
md5:61e39c114f8babc48c952b2ebf7f965f
8.7 MB Download
19
21
views
downloads
Views 19
Downloads 21
Data volume 183.7 MB
Unique views 17
Unique downloads 18

Share

Cite as