Published July 1, 2020 | Version 1.1
Dataset Open

CyclomicsSeq: Accurate detection of circulating tumor DNA using nanopore consensus sequencing

Creators

  • 1. Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Utrecht University, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands; Cyclomics, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands.
  • 2. Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Utrecht University, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
  • 3. Department of Genetics, University Medical Center Utrecht, Utrecht University, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
  • 4. Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands; Cyclomics, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands.

Description

CyclomicsSeq is a protocol designed to produce and sequence long DNA concatemers with a linear repetition to acquire high accuracy consensus reads. In this dataset, we used CyclomicsSeq for sequencing TP53 in cell-free DNA of healthy individuals and of head and neck cancer patients and for sequencing synthetic TP53 DNA sequences that mimic the length of cell-free DNA. This dataset contains data (mainly base calls of the backbone and the insert) of 32 nanopore sequencing runs. 
 

Notes

The Utrecht Sequencing Facility is subsidized by the University Medical Center Utrecht, Hubrecht Institute, and Utrecht University. This study was financially supported by the Oncode Institute (project number P2018-004) and is part of the Oncode Institute, which is partly financed by the Dutch Cancer Society.

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Cyclomics_manuscript.zip

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