Published June 17, 2020 | Version v1
Dataset Open

Progesterone Electron Diffraction (MicroED) Datasets - Glacios TEM with a CETA-D

  • 1. NanoImaging Services

Description

Electron diffraction datasets collected from nanometer sized crystals of progesterone (Sigma) applied to an electron microscopy grid covered in a pure carbon film. Data were collected using a Thermo Fisher Scientific Glacios Transmission Electron Microscope operated at 200 kV and -193 C using the software package Leginon. The beam diameter is ~600 nm and the total dose is ~6.8e-/A^2. The crystal was continuously rotated and diffraction data was collected with a CETA-D camera in rolling shutter mode. Data have been converted to SMV format with the addition of an offset value to remove negative pixel values. This offset value and a recommended pedestal value can be found in the image headers. An image of the crystal before centering on the small green dot is also included. Data from four crystals are provided, with 352382_1 being the best dataset. If processing in DIALS, you will first want to install the proper format class: 

dxtbx.install_format -u https://raw.githubusercontent.com/dials/dxtbx_ED_formats/master/FormatSMVCetaD_TUI.py

Files

352373_1.zip

Files (1.9 GB)

Name Size Download all
md5:e56371f4171ad4426bb9daf8d033f93c
462.5 MB Preview Download
md5:df20cbb7fdee659a6c0846694b11d78e
460.0 MB Preview Download
md5:971b54b308343acf492b48f8fa2fbb9d
466.7 MB Preview Download
md5:0d4118670056772ec17937f1aabe7ae1
469.6 MB Preview Download

Additional details

Related works

Is cited by
Journal article: 10.3389/fmolb.2020.00179 (DOI)
Dataset: 10.5517/ccdc.csd.cc27zbtd (DOI)