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Published January 27, 2021 | Version v1
Dataset Open

Tissue-mimics example datasets for Icy protocols

  • 1. RESTORE-GOT IT Team UMR 5070-CNRS 1301-INSERM EFS Univ. Toulouse, France
  • 2. ICFO-Institut de Ciences Fotonique, Av. Carl Friedrich Gauss, 3, Castelldefels, 08860, Barcelona, Spain

Description

This upload contains three image datasets to test the Icy protocols P1 to P3 and plugin metroloSPIM described in Andilla, J., Jorand, R., Olarte, O. et al. Imaging tissue-mimic with light sheet microscopy: A comparative guideline. Sci Rep 7, 44939 (2017). https://doi.org/10.1038/srep44939  

MCTS_H2B-FP.tif: tissue-mimics example dataset to test Icy protocol P1 – Detect tissue mimics and crop 3D sub-volume

MCTS_H2B-FP_crop.tif: crop of MCTS_H2B-FP.tif (tissue-mimics example dataset) to test Icy protocol P2 – SNR and contrast measurements 

MCTS_Beads.tif: fluorescent beads example dataset to test Icy protocol P3 – Characterize the PSF from beads in 3D 

Files

MCTS_Beads.tif

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Additional details

References

  • Andilla, J., Jorand, R., Olarte, O. et al. Imaging tissue-mimic with light sheet microscopy: A comparative guideline. Sci Rep 7, 44939 (2017). https://doi.org/10.1038/srep44939