Particle surfaces to study macrophage adherence, migration and clearance
Creators
- 1. Adolphe Merkle Institute
- 2. Helmholtz-Zentrum Dresden-Rossendorf
Description
These datasets are used to produce the figures/graphs published in our Advanced Functional Materials (10.1002/adfm.202002630) entitiled:
Particle surfaces to study macrophage adherence, migration and clearance
by
Dedy Septiadi, Aaron Lee, Miguel Spuch-Calvar, Thomas Lee Moore, Giovanni Spiaggia, Wildan Abdussalam, Laura Rodriguez-Lorenzo, Patricia Taladriz-Blanco, Barbara Rothen-Rutishauser, Alke Petri-Fink*
Nanoparticle adsorption to substrates pose a unique challenge to understand uptake mechanisms as it involves the organization of complex cytoskeletal components by cells to perform endocytosis/phagocytosis. In particular, it is not well-understood from a cell mechanics perspective how the adhesion of particles on substrate will influence the ease of material clearance. By using a particle model, we simulate and study key contributing factors underlying cell adhesion on non-porous silica particle surfaces, migration and engulfment. Following a 24 hour incubation period, monocyte-derived macrophages and A549 epithelial cells are able to adhere and remove particles in their local vicinity through induction of adhesive pulling arise from cell traction forces and phagocytic/endocytic mechanisms, in a size-dependent manner. We observe that such particle-decorated surfaces can be used to address the influence of surface topography on cell behavior. Substrates which presented 480 nm silica particles are able to induce greater development and maturation of focal adhesions, which play an important role in cellular mechanoregulation. Moreover, under a chemotactic influence, in the presence of 30% fetal bovine serum, macrophages are able to uptake the particles and be directed to translocate along a concentration gradient, indicating that local mechanical effects do not substantially impair normal physiological functions.
Notes
Files
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