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Sequencing Analysis Guide

Nicouleau, Michael; Durcan, Thomas M.

Sanger sequencing is a method of DNA sequencing that is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.

Sanger sequencing analysis software packages are used for base calling (process of assigning nucleobases to chromatogram peaks), sequence alignment, trace visualization and variant detection.

In this protocol, we describe our method used for analyzing sequence data obtained with our Applied Biosystems SeqStudio Genetic Analyzer instrument and analyzed using SnapGene software.

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