Published April 30, 2020
| Version v1
Dataset
Open
Fluorescent Macrophages in Drosophila Embryo
Creators
- 1. King's College London
- 2. City, University of London
Description
This is a data set that contains a time sequence of fluorescently labelled macrophages that migrated in a drosophila embryo.
The macrophages were visualised in the embryo by using the UAS/GAL4 system.We used the srpHemo-Gal4driver,which mediate the expression of genes downstream of a UASsequence specifically in macrophages to express the following UAS fluorescent probes: UAS-RedStinger for the nuclei and UAS-Clip-GFP for the microtubules.
Details of the imaging and preparation have been published in:
- Evans, I.R.; Zanet, J.; Wood, W.; Stramer, B.M. Live imaging of Drosophila melanogaster embryonic339hemocyte migrations.Journal of visualized experiments: JoVE2010,36.
Details on the segmentation, tracking and analysis of the macrophage migration have been published in:
- José Alonso Solís-Lemus, Besaiz J Sánchez-Sánchez, Stefania Marcotti, Mubarik Burki, Brian Stramer, Constantino Carlos Reyes-Aldasoro, Comparative study of contact repulsion in control andmutant macrophages using a novel interaction detection, Journal of Imaging, BioRxiv, https://doi.org/10.1101/2020.03.31.018267
and
- Solís-Lemus, J.A.; Stramer, B.; Slabaugh, G.; Reyes-Aldasoro, C.C. Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab.Journal of Imaging 2019,5
Files
Macrophages_Drosophila_2012_11_20_3.gif
Files
(562.8 MB)
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Additional details
References
- José Alonso Solís-Lemus, Besaiz J Sánchez-Sánchez, Stefania Marcotti, Mubarik Burki, Brian Stramer, Constantino Carlos Reyes-Aldasoro, Comparative study of contact repulsion in control andmutant macrophages using a novel interaction detection, BioRxiv, https://doi.org/10.1101/2020.03.31.018267
- Evans, I.R.; Zanet, J.; Wood, W.; Stramer, B.M. Live imaging of Drosophila melanogaster embryonic339hemocyte migrations.Journal of visualized experiments: JoVE2010, 36.