Preprint Open Access
Researchers at the Wuhan Institute of Virology (WIV) were using Human Immunodeficiency Virus (HIV-1) derived plasmids, cotransfecting them along with SARS-like virus derived spike gene plasmids, into human embryonic kidney (HEK) cells. This created pseudoviruses so that the experiment can be performed in a Biosafety Level 2 (BSL-2) laboratory instead of the BSL-4 required for SARS-like viruses. Their spike gene plasmid solution however, could of course be contaminated with SARS-like viruses due to inadequate purification, which infect the HEK cells. With HEK cells containing transfected HIV-1 genetic material, novel recombinant SARS-like viruses can be created with HIV-1 derived inserts. Such novel coronaviruses would have been exposed to laboratory workers and animals during handling, especially in a relaxed BSL-2 setting, thus resulting in SARS-CoV-2 and COVID-19.
Researchers identified the signature of 4 such HIV-1-like inserts in the SARS-CoV-2 spike protein. Two more inserts identified in the SARS-CoV-2 ORF1a protein are described here. All inserts have high homology to HIV-1 sequences.
Contamination is a fundamental problem that plagues drugs, vaccines, biologics, diagnostics and laboratory research materials. The biotechnology and pharmaceutical industries have done next to nothing about it. Egg, milk protein contaminated vaccines cause egg/milk allergy. Animal/plant/fungal protein contaminated vaccines cause the explosion of autoimmune disorders. Insulin injections are contaminated with yeast proteins which results in de novo autoimmune disorders. Rotavirus vaccines are contaminated with viable porcine circoviruses. Animal tissue and serum used in vaccine manufacturing are derived from animals infected by numerous diseases. And of course coronavirus infected animal material contaminating vaccines, cause IgE mediated sensitization against coronavirus proteins thus leading to COVID-19 severity. Products used in humans are supposed to be held to a “higher standard”. So what level of contamination can we expect in diagnostics and materials used in laboratory research?
The WIV was experimenting with numerous SARS-like coronaviruses (SARS-L-CoV). The HEK were grown using fetal calf serum. So their experiments were contaminated with SARS-L-CoV and its genetic material, HIV-1 and its genetic material, any bovine viruses in the fetal calf serum, human viruses in HEK and their genetic material. When a cell is infected with multiple viruses or contaminated with genetic material from multiple viruses, recombination can occur creating novel viruses. With millions of cells contaminated as above, the Wuhan laboratory (and other such laboratories) were incubators for novel viruses including coronaviruses such as SARS-CoV-2. The laboratories in China were also notorious for leaking viruses. So it was only a matter of time before a virus with human-to-human transmission capability would leak. It did, in November 2019.
Biosafety is a misnomer. These are biohazard laboratories. These laboratories, around the world, must be immediately shut down before they wipe out humanity with the next disease.