Journal article Open Access
Bob I.A. Mgbeje; James Ofem Otu; Flora O. Ugoanyanwu
Earlier studies have shown the butanol fraction of Vernonia amygdalina to be ypoglycaemic, hepatoprotective, and to ameliorate diabetes induced decreases in the level of male hormone and testicular damage. The aim of this study was to evaluate the phytochemical composition of the butanol fraction of V.amygdalina leaf extract using Gas Chromatography-Mass spectroscopy (GC-MS) with a view to unravelling the phytochemical constituents responsible for these antidiabetic activities. The GC-MS analysis of the butanol fraction revealed the presence of 18 major important bioactive compounds namely: 3, 5-bis 1, 1 dimethylethyl Phenol, Tetradecanoic acid, 1, 2-epoxyhexadecane (Oxirane), Methylhexadecanoate (Palmitic acid), Hexadecanoic acid (Eicosanoic acid), 9, 12-octadecadienoic acid (Linoleic acid), 3, 7-dimethyldodecan-1-ol (Phytol), 6-octadecenoic acid (Oleic acid), octadecanoic acid (Stearic acid), Cholest-5, 3-ol, 5-acetate (Cholestane), 1,2-Benzenedicarboxylic acid (Di-n-octyl phthalate), Hexadecanoic acid butyl ester, 16-Hexadecanoyl hydrazide, Decane, 1-fluoro- Decyl fluoride 1-(Fluorodecane),9,12-Octadecadien-1-ol, 9,12,15-Octadecatrien-1-ol, Di-n-octyl phthalate 1,2-(Benzenedicarboxylic acid, dioctyl ester), 2,6,10,14,18,22-Tetracosahexaene (or 2,6,10,15,19,23-hexamethyl [squalene]). Of the 18 phytochemical constituents, 14 are known to have a bearing on diabetes.