Published March 24, 2020
| Version v1
Dataset
Open
SEC-MALS: USP5 and Ubiquitin
- 1. University of Toronto, Structural Genomics Consortium
Description
Determination of the stoichiometry of binding of monoubiquitin (Ubq) to the full-length wildtype USP5 and R221A mutant protein by size exclusion chromatography-multiple angle light scattering (SEC-MALS) to elucidate the binding properties of native Ubq for future cell-based and activity studies.
Notes
Funding acknowledgement: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
Files
1_SECMALS.pdf
Files
(1.9 MB)
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