Published June 27, 2019 | Version v1
Journal article Open

A novel seed plants gene regulates oxidative stress tolerance in Arabidopsis thaliana

Authors/Creators

  • 1. BioAtlantis Ltd.
  • 2. Center of Plant Systems Biology and Biotechnology
  • 3. Institute of Biochemistry and Biology, University of Potsdam
  • 4. Department of Plant Breeding & Genetics, Faculty of Agricultural Sciences & Technology, Bahauddin Zakariya University
  • 5. Max Planck Institute of Molecular Plant Physiology
  • 6. Center of Plant Systems Biology and Biotechnology,Max Planck Institute of Molecular Plant Physiology
  • 7. Center of Plant Systems Biology and Biotechnology, Institute of Biochemistry and Biology, University of Potsdam, Max Planck Institute of Molecular Plant Physiology,
  • 8. Department of Molecular Pharmacology, University of Groningen
  • 9. Center of Plant Systems Biology and Biotechnology, Department of Plant Physiology and Molecular Biology, University of Plovdiv

Description

Oxidative stress can lead to plant growth retardation, yield loss, and death. The atr7 mutant of Arabidopsis thaliana exhibits pronounced tolerance to oxidative stress. Using positional cloning, confrmed by knockout and RNA interference (RNAi) lines, we identifed the atr7 mutation and revealed that ATR7 is a previously uncharacterized gene with orthologs in other seed plants but with no homology to genes in lower plants, fungi or animals. Expression of ATR7-GFP fusion shows that ATR7 is a nuclear-localized protein. RNA-seq analysis reveals that transcript levels of genes encoding abiotic- and oxidative stress-related transcription factors (DREB19, HSFA2, ZAT10), chromatin remodelers (CHR34), and unknown or uncharacterized proteins (AT5G59390, AT1G30170, AT1G21520) are elevated in atr7. This indicates that atr7 is primed for an upcoming oxidative stress via pathways involving genes of unknown functions. Collectively, the data reveal ATR7 as a novel seed plants-specifc nuclear regulator of oxidative stress response.

Notes

Published online: 27 June 2019

Files

A novel seed plants gene regulates oxidative stress tolerance.pdf

Files (1.7 MB)

Additional details

Funding

European Commission
PlantaSYST - Establishment of a Center of Plant Systems Biology and Biotechnology for the translation of fundamental research into sustainable bio-based technologies in Bulgaria 739582