Supplement of manuscript 521330
Creators
- 1. Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research
- 2. Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity
Description
This folder contains analysis data from the study submitted as manuscript (Manuscript ID 521330):
TITLE:
Comparative metabarcoding and metatranscriptomic analysis of microeukaryotes within coastal surface waters of West Greenland and Northwest Iceland
AUTHORS:
Stephanie Elferink, Stephanie.westphal@awi.de, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research
Sylke Wohlrab, Sylke.wohlrab@awi.de, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity
Stefan Neuhaus, Stephan.neuhaus@awi.de, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research
Allan Cembella, Allan.Cembella@awi.de, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research
Lars Harms, Lars.Harms@awi.de, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity
Uwe John, Uwe.John@awi.de, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity
JOURNAL:
Frontiers in Marine Science, section Marine Molecular Biology and Ecology
MS-ID:
521330
HOWTO:
Sequences identified as Alveloates or Stramenopiles (description in manuscript) had been were classified more accurately by PhyloAssigner version 6.166 (https://github.com/jungbluth/phyloassigner, Vergin et al., 2013, DOI:10.1038/ismej.2013.32) with a phylogenetic placement onto reference trees based on 18S/28S concatenated alignments, according to Elferink et al. 2017 (DOI: 10.1016/j.dsr.2016.11.002).
CONTENT:
reference databases:
- Alveolata_SSU-LSU-concat_310715_636.phyloassignerdb
- Stramenopiles_SSU_LSU_concat_030815_1777.phyloassignerdb
query sequence files:
- Alveolata_seqtab_SIGN_dada2.fasta
- Alveolata_seqtab_SIGN_dada2.fasta
created out put folder including the taxonomic annotation:
- Alveolata_seqtab_SIGN_dada2.place.out
- Stramenopiles_seqtab_SIGN_dada2.place.out
text file containing the used commands:
- commands
Files
Alveolata_seqtab_SIGN_dada2.place.out.zip
Files
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