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Published February 29, 2020 | Version v.1.0
Dataset Open

Supplement of manuscript 521330

  • 1. Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research
  • 2. Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity

Description

This folder contains analysis data from the study submitted as manuscript (Manuscript ID 521330):

TITLE:

Comparative metabarcoding and metatranscriptomic analysis of microeukaryotes within coastal surface waters of West Greenland and Northwest Iceland

AUTHORS:

Stephanie Elferink, Stephanie.westphal@awi.de, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research  

Sylke Wohlrab, Sylke.wohlrab@awi.de, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity

Stefan Neuhaus, Stephan.neuhaus@awi.de, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research  

Allan Cembella, Allan.Cembella@awi.de, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research  

Lars Harms, Lars.Harms@awi.de, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity

Uwe John, Uwe.John@awi.de, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity

JOURNAL:

Frontiers in Marine Science, section Marine Molecular Biology and Ecology

MS-ID:

521330

HOWTO:

Sequences identified as Alveloates or Stramenopiles (description in manuscript) had been were classified more accurately by PhyloAssigner version 6.166 (https://github.com/jungbluth/phyloassigner, Vergin et al., 2013, DOI:10.1038/ismej.2013.32) with a phylogenetic placement onto reference trees based on 18S/28S concatenated alignments, according to Elferink et al. 2017 (DOI: 10.1016/j.dsr.2016.11.002).

CONTENT:

reference databases:

- Alveolata_SSU-LSU-concat_310715_636.phyloassignerdb

- Stramenopiles_SSU_LSU_concat_030815_1777.phyloassignerdb

query sequence files:

- Alveolata_seqtab_SIGN_dada2.fasta

- Alveolata_seqtab_SIGN_dada2.fasta

created out put folder including the taxonomic annotation:

- Alveolata_seqtab_SIGN_dada2.place.out

- Stramenopiles_seqtab_SIGN_dada2.place.out

text file containing the used commands:

- commands

 

 

 

 

Files

Alveolata_seqtab_SIGN_dada2.place.out.zip

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