Published February 19, 2020 | Version v1
Journal article Open

BlOOD ANTIOXIDANT/OXIDANT PARAMETERS IN RATS EXPERIMENTALLY INFECTED WITH FASCIOLA HEPATICA AND EXPOSED TO CADMIUM

Creators

  • 1. Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov , Bulgarian Academy of Sciences, Sofia, Bulgaria

Contributors

  • 1. University of Forestry, Sofia, Bulgaria

Description

The aim of this study was to investigate antioxidant/oxidant status of blood of rats experimentally infected with Fasciola hepatica combined with chronic cadmium administration. Parameters of antioxidant/oxidant status were malondialdehyde (MDA), superoxide dismutase (CuZnSOD) and glutathione peroxidase (GPx) in blood of rats experimentally infected with Fasciola hepatica. The level of cadmium (Cd) was deter-mined as well. The data showed that oxidative/ antioxidative imbalance was developed due to an increased MDA level, reduced enzyme activity in double treated animals compared to control animals and these with only one treatment. Cd level was significantly increased in all rats received Cd (non-infected or infected with F. hepatica). Our study leads us to conclude that co-exposure to Cd and helminths causes a more pronounced increase in the blood oxidative stress in the hosts.

Files

vol-5-1_2020-48-52.pdf

Files (484.2 kB)

Name Size Download all
md5:59bff8f6ced233bfb44204ca441a98ba
484.2 kB Preview Download

Additional details

Related works

Is documented by
http://scij-tmvm.com/vol./vol.5/1/vol-5-1_2020-48-52.pdf (URL)

References

  • Anisimova, M., M. Gabrashanska. (2007). Antioxidant status in rats after Fasciola hepatica infec-tion and metal compounds treatment. XIV. Aktualne Problemy Humannej Parazitologie. Proceed-ings book. (Eds. R. Holkova, I. Tomo, A. Totkova, M. Klobusicky), Bratislava. 2007, 23–27.
  • Bottari, N., R. Mendes, N. Lucca, C. Schwertz, I. Henker, D. Olsson, A. S. Da Silva. (2015). Oxi-dative stress associated with pathological lesions in the liver of rats experimentally infected by Fac-iola hepatica. Experimental Parasitology, 159:24–28.
  • Erdem O., N. Yazihan, M. K. Kocak, A. Sayal, Ethem Akcil. (2016). Influence of chronic cadmium exposure on the tissue distribution of copper and zinc and oxidative stress parameters in rats. Toxicology and Industrial Health, 32(8): 1505–1514.
  • Facino, M., M. Carini, G. Aldini, R. Ceserani, I. Ceserani, Cavaletti, L. Vederio. (1993). Efficacy of glutathione for treatment of fascioliasis. An investigation in the experimentally infected rat. Drug Res, 43: 455–460
  • Hafeman D. G, Sunde R. A., Hoekstra W. G. (1974). Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. Journal of Nutr., 1974;104:580–7.
  • Jomova K., M. Valko. (2011). Advances in metal-induced oxidative stress and human disease. Tox-icology, 283 (2–3): 65–87.
  • Kolodziejczyk L, E. Siemieniu, E. Skrzydlewska. (19950. Antioxidant potential of rat liver in ex-perimental infection with Fasciola hepatica. Parasitol Res, 96: 367–372.
  • Londero D., P. Lo Greco. (1996). Automated high-performance liquid chromatographic separation with spectrofluorometric detection of malondyaldehyde-thiobarbituric acid adduct in plasma. Journal of Chromatography 729: 207–210.
  • Misra F., I. Fridovich. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247: 3170–3175.
  • Nzengue Y, S., Candeias, S., Sauvaigo. (2011). The toxicity redox mechanisms of cadmium alone or together with copper and zinc homeostasis alteration: its redox biomarkers. Journal of Trace elements in Medicine and Biology, 25: 171–180.
  • Renugadevi J., S. M. Prabu. (2010). Cadmium –induced hepatotoxicity and renal toxicity and pro-tective effect of naringenin. Experimental Toxicology and Pathology, 62: 171–181.
  • Valko M, M. Morris, M. Cronin. (2005). Metals, toxicity and oxidative stress. Curr. Med. Chem. 12 (10): 1161:1208.