IMMUNOMODULATION OF LACTOBACILLUS PLANTARAM EXPRESSING PORCINE ROTAVIRUS VP6
- 1. College of Animal Science and Technology Jilin University, Changchun, 13018.
- 2. Ministry of Agriculture, Livestock and Fisheries, P.O. Box 9152, Veterinary Complex, 131 Nelson Mandela Rd, Dar es Salaam, Tanzania.
Description
Rotaviruses are members of the family Reoviridae. Depending on their particular inner capsid protein sequences, porcine rotaviruses are categorized into groups A, B and C. Lactic acid bacteria (LAB) are the common term for bacteria that produce a large amount of lactic acid and ferment carbohydrates. We constructed a Lactobacillus plantarum strain with surface displayed VP6, named NC8-pSIP409-pgsA-VP6-Dcpep The adhesion and invasion ratios of NC8-pSIP409-pgsA-VP6-Dcpep on BMDCs were 36.17% and 2.23%, respectively,which were 1.56 fold and 2.42 fold increased compared with empty vector group. Furthermore, NC8-pSIP409-pgsA-VP6-Dcpep strain harboring a eukaryotic plasmid pValac-GFP was co-cultured with BMDCs for 36h and GFP expression in BMDCs was detected by FCM to determine the delivery efficiency of pValac-GFP. The results showed that the strain could significantly enhance GFP expression in BMDCs (P=0.0110). The mice were immunized using the above strains harboring pValac-GFP and the oral administration resulted in efficient expression of GFP in both PPs andMLN at different times after the last administration. In addition, the NC8-pSIP409-pgsA-VP6-Dcpep strain significantly promoted the differentiation and maturation of DCs, the percentages of IL-4+and IL-17A+ of CD4+T cells in MLN, as well as production of B220+ IgA+ B cells in the PPs.
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