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Online data set of "A protocol to quantify cellular morphodynamics: from cell labelling to automatic image analysis"

Manich Maria

Cellular morphodynamics can be used as markers for many physiological and pathological processes. This protocol provides a step-by-step guide to identify variations in motility and morphology within (or across) cell populations using non-invasive live imaging and reproducible image analysis techniques such as segmentation and tracking. Detailed instructions cover all the way from cell culturing and labelling to automatic image and statistical analyses, including the definition of multiple descriptors that characterise the shape and movement of cells in a quantitative manner. All methods are available as free open-source software and illustrated by video tutorials.

 

Files (5.3 GB)
Name Size
221219-ICY-MOVIES - 25X 29.tif
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98.8 MB Download
221219-ICY-MOVIES - 25X 29.xml
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3.2 kB Download
221219-ICY-MOVIES - 25X 30.tif
md5:6fa87cf4da518812cc57d0c43caa4b4b
89.3 MB Download
221219-ICY-MOVIES - 25X 30.xml
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3.6 kB Download
221219-ICY-MOVIES - 25X 32.tif
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181.8 MB Download
221219-ICY-MOVIES - 25X 32.xml
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2.3 kB Download
221219-ICY-MOVIES - 25X 33.tif
md5:38d3c87d5d15c3e7069f6156390677f8
194.9 MB Download
221219-ICY-MOVIES - 25X 33.xml
md5:14bf76056916c9aec545956b57bba438
2.3 kB Download
221219-ICY-MOVIES - 25X 34.tif
md5:ab76efff192f8bc22a55a58b115a64aa
84.5 MB Download
221219-ICY-MOVIES - 25X 34.xml
md5:da3f9c8a3c9b04cb0750613f58420e77
2.3 kB Download
221219-ICY-MOVIES - 25X 35.tif
md5:b4109d1281f0a5c78b36676362df35eb
46.3 MB Download
221219-ICY-MOVIES - 25X 35.xml
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3.6 kB Download
221219-ICY-MOVIES - 25X 36.tif
md5:5393a42ae79ef5a11ff6cfed70a89b14
220.6 MB Download
221219-ICY-MOVIES - 25X 36.xml
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3.2 kB Download
221219-ICY-MOVIES - 25X 38.tif
md5:501a7141a6b1181656d06483628a3820
125.7 MB Download
221219-ICY-MOVIES - 25X 38.xml
md5:9dd7f599a0393becb3223bd9878e9280
2.7 kB Download
221219-ICY-MOVIES - 25X 40.tif
md5:18f7faabc990fa935d618ecde0ab9c3f
163.7 MB Download
221219-ICY-MOVIES - 25X 40.xml
md5:63b5cb8ec491d6ad3fdd984588d80059
6.3 kB Download
221219-ICY-MOVIES - 25X 44.tif
md5:e5c972e253b5c88569ac7a6d022450fa
105.8 MB Download
221219-ICY-MOVIES - 25X 44.xml
md5:2e5d055ac2d7b6109034c0083c2e8c09
4.0 kB Download
221219-ICY-MOVIES - 25X 47.tif
md5:0f183ae7c6753ed1970c6db3fb41cc5c
155.3 MB Download
221219-ICY-MOVIES - 25X 47.xml
md5:aeaec5a869ca7980812f5b26d1c6b3bf
2.3 kB Download
221219-ICY-MOVIES - 25X 49.tif
md5:6ba8b920fad384eec218a3a6d2ba9e94
142.9 MB Download
221219-ICY-MOVIES - 25X 49.xml
md5:e7a4c12846b7e0542d163969214d0a7d
2.3 kB Download
221219-ICY-MOVIES - 25X 54.tif
md5:b2834a13f574d4e80508f1fe4cb6bf1d
167.2 MB Download
221219-ICY-MOVIES - 25X 54.xml
md5:a44c2721b7b8358d2c4ff8d2ad4bda40
2.3 kB Download
Mov.1-Fluorescent cells moving freely.avi
md5:831bd9695d301f28b57573c3720a7a34
215.5 MB Download
Mov.2-Segmented cells with Active Contours.avi
md5:ec6e00bbe92f434b32429e902cea36fa
215.5 MB Download
Mov.3-Segmented cells with Active Contours and centroid tracks.avi
md5:d74073c320c0a3878638761e7467acd8
215.5 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 29' view.avi
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122.7 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 30' view.avi
md5:6c4bc0c604ec64a9a12bf98204d0b2a0
150.9 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 32' view.avi
md5:2b9c6e3748c9765f865f2ec3e2345b02
227.3 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 33' view.avi
md5:6126362636c1fc601af43b9eaac6ae5e
243.8 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 34' view.avi
md5:074f5c6ceec4d2c3c09bfb39d356aa63
109.3 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 35' view.avi
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57.4 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 36' view.avi
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272.9 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 38' view.avi
md5:88b668b1cb1ee42190d7cb3da5f91a3f
155.7 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 40' view.avi
md5:5ad73405fbb7a7b570155401e8c5017f
215.5 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 44' view.avi
md5:f12d3f57417733f27bd09b2c12b90112
129.8 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 47' view.avi
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194.3 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 49' view.avi
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180.9 MB Download
Screen shot of '221219-ICY-MOVIES - 25X 54' view.avi
md5:0a427bea499c1ff6efff8345eadd12f0
209.2 MB Download
Tut. 1- Using Time Stamp Overlay plugin.mov
md5:1a1b006f5871a4fc3a32974494614b3a
34.8 MB Download
Tut. 2- HK-Mean segmentation on frame 0 and color ROIs affectation.mov
md5:e3c44cf61105744e95a8498ab4459737
23.6 MB Download
Tut. 3- Approximative ROIs manual design and automatic segmentation with Active Contours.mov
md5:6baaabcee856b01187e30740c8269d02
173.6 MB Download
Tut. 4- Automatic ROIs detection with HKM and segmentaion with Active Contours.mov
md5:5d5d9a42fa90f19ffc8df9249a03d8b2
46.0 MB Download
Tut. 5- Segmentation with Active Contours and cell tracks analysis with Track Manager and Track Processors.mov
md5:4e1344fdfd427217826dd703da113d4b
325.5 MB Download
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