IDENTIFICATION OF INULIN PROFILE FROM RED FRUIT (PANDANUS CONOIDEUS L) PEDICEL EXTRACT USING LC-MS AND ITS IN VITRO PREBIOTIC ACTIVITY TEST

Pedicel is a by-product of red fruit oil production. Identify the profile of inulin using LCMS (liquid chromatography-mass spectrometry) extracted from red fruit pedicels with conventional methods and hydrodynamic cavitation and testing their prebiotic activity. The results showed that the filtrate extracted from red fruit pedicels contained inulin compounds, evidenced by the presence of glucopyranoside and fructofuranosyl monomers associated with β bonds as markers. The chromatogram peak of the filtrate by conventional methods at retention time of 9.77 minutes had a base peak m/z of 686.740 g/mol with the alleged molecular formula C30H54O17, while the hydrodynamic cavitation method at 7.42 minutes retention time had a base peak m/z of 668.597 g/mol with the alleged molecular formula C30H36O17. The polymerization degree of inulin with both extraction methods was 4. In vitro test showed that the inulin in the red fruit pedicel extract supported the growth of the Lactobacillus casei colony. The number of colonies in the extract of the hydrodynamic cavitation method was 9.09 log CFU/ml higher than the conventional method, which was 8.89 log CFU/mL at 48 hours incubation time. The highest prebiotic activity value in the filtrate media of the hydrodynamic cavitation method was 0.9 at 48 hours incubation