Data related to the article "Spatio-temporal dynamic monitoring of fatty acid-receptors interaction on single living cells by multiplexed Raman imaging".

Spatio-temporal dynamic monitoring of fatty acid-receptors interaction on single living cells by multiplexed Raman imaging

Wei Zhang, Fangjun Lin, Yan Liu, Han Zhang, Timothy A. Gilbertson, Anhong Zhou

Data related to the article “Spatio-temporal dynamic monitoring of fatty acid-receptors interaction on single living cells by multiplexed Raman imaging”

The package includes raw data:

1. Calcium imaging data for Figure 2.

• Raw data_Calcium image_HEK293-GPR120.xls
• Raw data_Calcium image_HEK293-CD36.xls
• Raw data_Calcium image_TBDc1.xls

2. Raman real-time point measurement for Figure 3 (first 6 min upon LA addition) and Figure S3 (HEK293-GPR120 control experiments), Figure S4 (HEK293-CD36 control experiments), Figure S5 (TBDc1 control experiments).

• Raw data_Real time point_Figure 3(A)_HEK293-GPR120_LA.xls
• Raw data_Real time point_Figure 3(B)_HEK293-CD36_LA.xls
• Raw data_Real time point_Figure 3(C)_TBDc1_LA.xls
• Raw data_Real time point_Figure S3(A)_HEK293-GPR120_Control_PBS.xls
• Raw data_Real time point_Figure S3(B)_HEK293-GPR120_Control_MBA-, DTNB-NRs.xls
• Raw data_Real time point_Figure S3(C)_HEK293-GPR120_Control_No DOX.xls
• Raw data_Real time point_Figure S4(A)_HEK293-CD36_Control_PBS.xls
• Raw data_Real time point_Figure S4(B)_HEK293-CD36_Control_MBA-, DTNB-NRs.xls
• Raw data_Real time point_Figure S4(C)_HEK293-CD36_Control_No DOX.xls
• Raw data_Real time point_Figure S5(A)_TBDc1_PBS.xls
• Raw data_Real time point_Figure S5(B)_TBDc1_MBA-, DTNB-NRs.xls

3. Raman real-time mapping images for Figure 4 (receptor distribution upon addition of LA), Figure S6 (receptor distribution upon addition of MA), Figure S7 (receptor distribution upon addition of DHA).

• folder (HEK293-GPR120_LA_Figure 4)
• folder (HEK293-GPR120_MA_Figure S6)
• folder (HEK293-GPR120_DHA_Figure S7)
• folder (HEK293-CD36_LA_Figure 4)
• folder (HEK293-CD36_MA_Figure S6)
• folder (HEK293-CD36_DHA_Figure S7)
• folder (TBDc1_LA_Figure 4))
• folder (TBDc1_MA_Figure S6)
• folder (TBDc1_DHA_Figure S7)

4. Raman measurements of fatty acid internalization for Figure 5 (SERS responses to LA pretreated 5 min, then 24 hr incubation with SERS probes), Figure S8 (SERS responses to CA pretreated 5 min, then 24 hr incubation with SERS probes), Figure S9 (SERS responses to GW9508 pretreated 5 min, then 24 hr incubation with SERS probes).

• Raw data_Internalization_Figure 5(A)_HEK293-GPR120_LA.xls
• Raw data_Internalization_Figure 5(B)_HEK293-CD36_LA.xls
• Raw data_Internalization_Figure 5(C)_TBDc1_LA.xls
• Raw data_Internalization_Figure S8(A)_HEK293-GPR120_CA.xls
• Raw data_Internalization_Figure S8(B)_HEK293-CD36_CA.xls
• Raw data_Internalization_Figure S8(C)_TBDc1_CA.xls
• Raw data_Internalization_Figure S9 (A)_HEK293-GPR120_GW9508.xls
• Raw data_Internalization_Figure S9(B)_HEK293-CD36_GW9508.xls
• Raw data_Internalization_Figure S9(C)_TBDc1_GW9508.xls

5. Raman measurement of primary taste cells data for Figure 6. 

• Raw data_Primary cell_CV_LA.xls
• Raw data_Priary cell_FF_LA.xls