Chemical composition, antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts

The content of phenolic compounds (TPC) and glucans, as well as the effectiveness of antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts, were determined and compared. The content of glucans (total, α- and β-) strongly depended on the extraction time and particle size, but only the interaction of these parameters influenced the TPC. Gallic acid, quercetin, trans-cinnamic acid, kaempferol, hesperetin and naringenin were detected in extracts by HPLC–DAD. The most abundant phenols were hesperetin (1.875–3.222 µg/g) and naringenin (1.235–2.856 µg/g). The ethanol extracts exhibited noteworthy antioxidant activity, but the significant amount of phenolic compounds was strongly linked to polysaccharides, and hence reduced their antioxidant capacity. The results of the antiproliferative activity in vitro showed that the analyzed extracts were the most effective against HeLa cells. Significant correlations were observed between the antiproliferative effect and the TPC/glucan content of extracts.