Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)

Del Cid Pellitero, Esther; Shlaifer, RIna; Luo, Wen; Krahn, Andrea; Nguyen-Renou, Emmanuelle; Maneca, Destiny-Love; Rao, Trisha; Beitel, Lenore; Durcan, Thomas M.

doi:10.5281/zenodo.3385345

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Published September 4, 2019 | Version V1.0

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Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)

1. MNI, McGill University

This protocol describes how to:
• Determine fibril length of sonicated unlabelled α-synuclein preformed fibrils (PFFs) by dynamic light scattering (DLS) and electron microscopy (EM)
• Confirm cellular uptake of fluorescently-labelled α-synuclein PFFs by immunofluorescence (IF)

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EDDU-007-01_QC Characterization of a-syn PFFs_v1.0.pdf

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Technical note

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English

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Technical metadata

Created: September 4, 2019
Modified: July 22, 2024

Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)

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Files

EDDU-007-01_QC Characterization of a-syn PFFs_v1.0.pdf

Files (1.6 MB)