Dataset Open Access

The Tangential Nucleus Controls a Gravito-inertial Vestibulo-ocular Reflex

Bianco, Isaac; Ma, Leung-Hang; Schoppik, David; Robson, Drew; Orger, Michael; Beck, James; Li, Jennifer; Schier, Alexander; Engert, Florian; Baker, Robert

Background

Although adult vertebrates sense changes in head position by using two classes of accelerometer, at larval stages zebrafish lack functional semicircular canals and rely exclusively on their otolithic organs to transduce vestibular information.

Results

Despite this limitation, we find that larval zebrafish perform an effective vestibulo-ocular reflex (VOR) that serves to stabilize gaze in response to pitch and roll tilts. By using single-cell electroporations and targeted laser ablations, we identified a specific class of central vestibular neurons, located in the tangential nucleus, that are essential for the utricle-dependent VOR. Tangential nucleus neurons project contralaterally to extraocular motoneurons and in addition to multiple sites within the reticulospinal complex.

Conclusions

We propose that tangential neurons function as a broadband inertial accelerometer, processing utricular acceleration signals to control the activity of extraocular and postural neurons, thus completing a fundamental three-neuron circuit responsible for gaze stabilization.

Highlights

► The utricle and its associated circuitry drive a broadband vestibular-ocular reflex ► Linear summation of bilateral utricular signals mediates compensatory eye rotations ► Central vestibular neurons in the tangential nucleus are necessary for the VOR ► Visual and vestibular signals interact to enhance gaze stabilization

Files (3.3 GB)
Name Size
AnalyzedSteps.zip
md5:f21e5176191809d3e4a05c149272154d
58.7 MB Download
Code.zip
md5:16400e597e3eb5215e138a70fd0f3e4d
81.8 kB Download
processed.zip
md5:9f7b0d9ee2821d9ad4138ea8eb6cd2d8
910.8 kB Download
rawdata.zip
md5:e897fad62b25708dabe872a1da90976b
3.2 GB Download
rawDataSteps.zip
md5:36b3ec093e4a686ae6de3e6b858717cd
41.7 MB Download
README.txt
md5:f581e74f7bd790adc4d67d5e753b3f16
546 Bytes Download
StepFigures.zip
md5:fc9e0ed69b03946adfff6fa7e30c5461
1.0 MB Download
vestibdb.csv
md5:fa22c231e27936619a33f15a9aa16b74
72.0 kB Download
4
33
views
downloads
All versions This version
Views 44
Downloads 3333
Data volume 9.9 GB9.9 GB
Unique views 33
Unique downloads 44

Share

Cite as