Dysfunction telomeres in embryonic fibroblasts and cultured in vitro pluripotent stem cells of Rattus norvegicus (Rodentia, Muridae)
Creators
- 1. The Federal Research Center Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
- 2. The Federal Research Center Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia|Ministry of Health of the Russian Federation, Novosibirsk, Russia|Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia
Description
We studied the level of spontaneous telomere dysfunction in Rattus norvegicus (Berkenhout, 1769) (Rodentia, Muridae) embryonic fibroblasts (rEFs) and in cultured in vitro rat pluripotent stem cells (rPSCs), embryonic stem cells (rESCs) and induced pluripotent stem cells (riPSCs), on early passages and after prolonged cultivation. Among studied cell lines, rESCs showed the lowest level of telomere dysfunction, while the riPSCs demonstrated an elevated level on early passages of cultivation. In cultivation, the frequency of dysfunctional telomeres has increased in all studied cell lines; this is particularly true for dysfunctional telomeres occurring in G1 stage in riPSCs. The obtained data are mainly discussed in the connection with the specific structure of the telomere regions and their influence on the differential DNA damage response in them.
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34732__1_210286_LE_321806.pdf
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