Lipid–peptide bioconjugation through pyridyl disulfide reaction chemistry and its application in cell targeting and drug delivery
Creators
- 1. Dto. Química Física, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040, Madrid, Spain
- 2. Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 689-798, Republic of Korea
- 3. Instituto de Investigación Hospital Doce de Octubre (i+12), Avenida de Córdoba s/n, 28041, Madrid, Spain
- 4. Dto. Química Orgánica, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040, Madrid, Spain
- 5. Institut de Chimie Séparative de Marcoule, ICSM, UMR 5257, Site de Marcoule-Bât, 426 BP 17 171, 30207, Bagnols sur Ceze, France
Description
Background: The design of efficient drug delivery vectors requires versatile formulations able to simultaneously direct a multitude of molecular targets and to bypass the endosomal recycling pathway of cells. Liposomal-based vectors need the decoration of the lipid surface with specific peptides to fulfill the functional requirements. The unspecific binding of peptides to the lipid surface is often accompanied with uncontrolled formulations and thus preventing the molecular mechanisms of a successful therapy.
Results: We present a simple synthesis pathway to anchor cysteine-terminal peptides to thiol-reactive lipids for adequate and quantitative liposomal formulations. As a proof of concept, we have synthesized two different lipopeptides based on (a) the truncated Fibroblast Growth Factor (tbFGF) for cell targeting and (b) the pH sensitive and fusogenic GALA peptide for endosomal scape.
Conclusions: The incorporation of these two lipopeptides in the liposomal formulation improves the fibroblast cell targeting and promotes the direct delivery of cargo molecules to the cytoplasm of the cell.
Files
12951_2019_Article_509.pdf
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