Dataset Open Access

Optimisation of viability assay for DIPG patient-derived cell lines

Wong, Jong Fu; Bullock, Alex

Evaluation of the efficacy of M4K compounds in DIPG patient-derived cell lines is essential before any promising compounds can be further tested in mouse xenograft models. This approach can aid in narrowing down clinical compound candidates and reduce the time, resources and animal sacrifice needed downstream.

A robust and efficient readout for the changes in the viability of the DIPG cells needs to be established before it can be used to evaluate the M4K compounds. In addition, the amount of cells to be seeded at the beginning of the experiment has to be optimised to avoid overcrowding and starvation of the cells after extended culture times. Overcrowding and starvation will lead to increased cell death and prevent accurate estimation of the potency of M4K compounds (EC50).

Files (722.2 kB)
Name Size
Zenodo post014.pdf
md5:5e5eadc98eaf3ca419a57f92ab3261d9
722.2 kB Download
203
51
views
downloads
All versions This version
Views 203203
Downloads 5151
Data volume 36.8 MB36.8 MB
Unique views 181181
Unique downloads 4545

Share

Cite as