Journal article Open Access

DEVELOPMENT AND VALIDATION OF NEW ANALYTICAL METHOD FOR THE SIMULTANEOUSS ESTIMATION OF PANTOPRAZOLE AND DOMPERIDONE BY UV-SPECTROPHOTOMETRY

Barri Swathi*, B.Subramanyam, B.Dharmateja, M.Hari Babu, D.Kiran

A simple, rapid and precise method is developed for the quantitative simultaneous determination of Pantoprazole and Domperidone in combined pharmaceutical-dosage forms. The method was based on UV-Spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 290 nm (λmax of Pantoprazole) and 287 nm (λmax of Domperidone) in MeOH:ACN(60:40v/v). For UV Spectrophotometric method, linearity was obtained in concentration range of 1-15 μg/ml for domperidone and 1-50 μg/ml for Pantoprazole respectively, with regression 0.999 and 0.995 for Domperidone and Pantoprazole respectively. Recovery was in the range of 99 -103%; the value of standard deviation and %R.S.D were found to be < 2 %; shows the high precision of the method., in accordance with ICH guidelines. The method has been successively applied to pharmaceutical formulation and was validated according to ICH guidelines.

Files (752.9 kB)
Name Size
190307.pdf
md5:21f51c2d620126c47a61daa588c23d16
752.9 kB Download
48
81
views
downloads
All versions This version
Views 4848
Downloads 8181
Data volume 61.0 MB61.0 MB
Unique views 4747
Unique downloads 7171

Share

Cite as