Dataset Open Access
Harding, Rachel;
Alvarez, Claudia;
McAuley, Jacob;
Hutchinson, Ashley;
Seitova, Alma;
Arrowsmith, Cheryl;
Edwards, Aled
Project: Biophysical investigation of purified HTT protein samples
Experiment: Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography
Date completed: 2019/05/22
Rationale: Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In small-scale experiment, I also incorporated helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment included 3 affinity chromatography steps with FLAG, heparin and NiNTA resin and finally a gel filtration step and yielded a highly pure HTT-HAP40 sample: https://zenodo.org/record/3234174.I now want to try this purification with apo HTT.
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Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 20190522.docx
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Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 20190522.pdf
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