Dataset Open Access
Harding, Rachel;
Deme, Justin;
Lea, Susan;
Arrowsmith, Cheryl;
Edwards, Aled
Project: High resolution structural analysis of purified HTT samples
Experiment: Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation
Date completed: 2019/04/15
Rationale: We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by cryoEM.
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Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation_20190415.docx
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Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation_20190415.pdf
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