Poster Open Access

Microbiological transformation of enrofloxacin by the fungus Mucor ramannianus

Parshikov, I.A.; Freeman, J.P.; Lay, J.O.Jr.; Beger, R.D.; Williams, A.J.; Sutherland, J.B.

Fluoroquinolones are synthetic antimicrobial agents that are active against a broad spectrum of pathogenic gram-negative bacteria as well as some gram-positive bacteria and mycoplasmas. Several fluoroquinolones are used in clinical medicine. The metabolism of enrofloxacin by Mucor ramannianus was investigated as a model for the biotransformation of veterinary fluoroquinolones by zygomycetous fungi. Cultures grown at 28˚C in 500 ml flasks containing 100 ml of a sucrose-peptone broth on a rotary shaker at 200 rpm. After 2 days the culture was dosed with enrofloxacin in concentration 100 mg/L. After 21 more days the mycelia was separated by filtration. The culture fluid was extracted with methylene chloride in a separatory funnel and solvent was evaporated in vacuo. The products were analysed by HPLC. 22% of the original enrofloxacin remained. Three metabolites were purified by high-performance liquid chromatography and initially characterized by the UV/visible absorption spectra. They were identified by ESI/MS/MS and ESI/LC/MS mass and nuclear magnetic resonance spectrums.

Files (379.2 kB)
Name Size
379.2 kB Download
All versions This version
Views 4141
Downloads 6565
Data volume 24.7 MB24.7 MB
Unique views 4141
Unique downloads 6565


Cite as