Published May 6, 2019
| Version v1
Dataset
Open
NIH3T3_point_locations for RNA seqFISH+ experiments
Description
These source data consist of the point locations of individual decoded mRNA dots in seqFISH+ experiments on NIH/3T3 cells. DAPI_experiment folder contains the DAPI stainings with other beads images. ROIs_experiment folders are the manual segmentation performed in ImageJ. We only performed segmentation on the whole single cells. In seqFISH+_NIH3T3_point_locations zip folder, there are 3 matlab files . One is gene name file, the other two files are point locations for replicate 1 and 2 seqFISH+ experiments in NIH/3T3 cells. The columns of the data represent field of view(FOV), Cell , and Gene. For example : FOV1 , Cell 5, Gene : Bgn to retrieve the point locations.
Files
DAPI_experiment1.zip
Files
(772.2 MB)
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md5:2ab21edfa5788a717829d94656393524
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Additional details
References
- Eng, C.-H. L. et al. Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH+. Nature 568, 235–239 (2019)