Dataset Open Access
NIH3T3_point_locations for RNA seqFISH+ experiments
Chee Huat (Linus) Eng; Long Cai
These source data consist of the point locations of individual decoded mRNA dots in seqFISH+ experiments on NIH/3T3 cells. DAPI_experiment folder contains the DAPI stainings with other beads images. ROIs_experiment folders are the manual segmentation performed in ImageJ. We only performed segmentation on the whole single cells. In seqFISH+_NIH3T3_point_locations zip folder, there are 3 matlab files . One is gene name file, the other two files are point locations for replicate 1 and 2 seqFISH+ experiments in NIH/3T3 cells. The columns of the data represent field of view(FOV), Cell , and Gene. For example : FOV1 , Cell 5, Gene : Bgn to retrieve the point locations.
| Name | Size | |
|---|---|---|
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DAPI_experiment1.zip
md5:2ab21edfa5788a717829d94656393524 |
267.0 MB | Download |
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DAPI_experiment2.zip
md5:77a5b6cc17049b2e086bc8c5375ebc0d |
369.4 MB | Download |
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ROIs_Experiment1_NIH3T3.zip
md5:75b7b62c79014fc7e171a692beb129b8 |
154.8 kB | Download |
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ROIs_Experiment2_NIH3T3.zip
md5:66dc9f6c6f2e9c5478eb64e54e7bce87 |
135.2 kB | Download |
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seqFISH+_NIH3T3_point_locations.zip
md5:a228f8e6f64b0506ce072a5e6d469038 |
135.4 MB | Download |
Eng, C.-H. L. et al. Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH+. Nature 568, 235–239 (2019)
| All versions | This version | |
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- Publication date:
- May 6, 2019
- DOI:
-
- Published in:
- Nature:.
- License (for files):
- Creative Commons Attribution 4.0 International