Dataset Open Access

Optimisation of HTT-HAP40 purification using heparin affinity chromatography - 2019/04/02

Harding, Rachel; Arrowsmith, Cheryl; Edwards, Aled

Project: Biophysical investigation of purified HTT protein samples

Experiment: Optimisation of HTT-HAP40 purification using heparin affinity chromatography

Date completed:­ 2019/04/02

Rationale: Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In addition, I will incorporate helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment will include 3 affinity chromatography steps with FLAG, heparin and NiNTA resin and finally a gel filtration step. 

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. Dr. Harding's research is also supported with generous funding from the Huntington Society of Canada and the CHDI Foundation. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
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