Published July 1, 2014 | Version v1
Journal article Open

STUDY ON THE EFFICACY OF SYNBIOTICS IN THE PREVENTION OF SALMONELLA TYPHIMURIUM IN CHICKENS

  • 1. Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

Description

One hundred and twenty (day-old Cubb) chicks were equally divided into 6 groups (20 chicks per each).Group (1) kept as control negative(non treated), Group (2,5) treated with lactic acid (1% in drinking water in 1st week then decreased to 0.5 % all over the experimental period), Group( 3,6) treated with symbiotic (1x108 CFU in drinking water during entire period of rearing) Group 4, 5 and 6 were orally infected with  S. Typhimurium (Streptomycin-resistant strain) with infective dose 1X108 CFU at 4th day old. Quantitative and qualitative bacterial colonization were reduced in treated and infected groups. Poultry Star®   showed higher reduction colonization rate followed by Lactic acid compared to non-treated group. Hemaglutination-inhibition test (HI) against Newcastle disease (ND) vaccines showed an increase in the antibody titers in Poultry Star® treated groups (3, 6). Furthermore Poultry Star®was capable of enhancing performance, decreasing re-isolation rate of S. Typhimurium either from cloacal swaps and/or from (Liver and spleen). It could be conclude that Synbiotic and organic acid have great value on poultry production as growth promoter by either enhancing performance or reduction the intestinal colonization with S. Typhimurium as a model of pathogenic bacteria and improving the immune response.

Files

Eldemerdash_et_al.pdf

Files (674.1 kB)

Name Size Download all
md5:b7769155fa2a630715fb551e78292c8a
674.1 kB Preview Download

Additional details

References

  • Ali, A.S.; Amineh, M.; Parvin, S.and Mehdi, A. (2013): Immune responses to dietary inclusion of prebiotic-based mannan-oligosaccharide and β-glucan in broiler chicks challenged with Salmonella enteritidisTurk J Vet AnimSci(2013) 37: 206-213.
  • Ashayerizadeh,A.; Dabiri,N.; Ashayerizadeh,O.; Mirzadeh,K.H.; Roshanfekr,H and Mamooee, M. (2009): Effect of dietary antibiotic, probiotic and prebiotic as growth promoters, on growth performance, carcass characteristics and hematological indices of broiler chickens.Pak.J.Biol. Sci.12 (1): 52-57.
  • Awad, W.A.; Ghareeb, K. and Böhm, J. (2011): Evaluation of the chicory inulin efficacy on ameliorating the intestinal morphology and modulating the intestinal electrophysiological properties in broiler chickens. J. Anim. Physiol. Anim. Nutr., 95: 65-72.
  • Barrow, P. A.; Huggins, M. B.; Lovell, M. A. and Simpson, J. M. (1987): Observations on the pathogenesis of experimental Salmonella typhimurium infection in chickens. Res. Vet. Sci. 42:194–199.
  • Bjerrum, L., Engperg, R.M and Pedersen. K (2003): Infection models for Salmonella Typhimurium DT110 in day –old and 14-day old broiler chickens kept isolators. Avian Dis.47:1474-1480.
  • Byrd, J. A.; Hargis, B. M.; Caldwell, D. J.; Bailey, R. H.; Herron, K. L.; McReynolds, J. L.; Brewer.; Anderson, R. C.; Bischoff, K. M.; Callaway, T. R. and Kubena. L. F. (2001): Effect oflactic acid administration in the drinking water duringpreslaughter feed withdrawal on Salmonella andCampylobactercontamination of broilers. Poult.Sci. 80:278- 283.
  • Chalghoumi, R. C.; Marcq, A.; Thewis, D. Portetelle, and Y. Beckers.(2009): Effects of feed supplementation with specific hen eggyolk antibody (immunoglobulin Y) on Salmonella species cecalcolonization and growth performances of challenged broiler chickens. Poult. Sci. 88:2081–2092.
  • Corcionivoschi, N.; Drinceanu, D.; Pop, I. M.; Stack, D.; Stef, L.; Călin, J.; Bourke, B.( 2010):The effect of probiotics on animal health, Scientific Papers Animal Science and biotechnologies, , 43, 35-41
  • Cunningham, C. H. (1971): VirologiaPractica. 6thed.EditoraAcribia, Saragossa, Spain.
  • Dahiya, N.; Tewari, R.; Hoondal, G.S. (2006): Biotechnological aspects of chitinolytic enzymes: a review. Appl. Microbiol. Biotechnol. 71, 773–782.
  • Doyle, M.E. (2003): Alternatives to antibiotic use growth promotion in Animal Husbandry Food Research Institute. University of Wisconsin.
  • Edens, F.W. (2003): An alternative for antibiotic use in poultry: probiotics. RevistaBrasileira de CiênciaAvícola, 5:75-79.
  • Eeckhaut, V.; Van Immerseel, F.;Dewulf, J.;Pasmans,F.;Haesebrouck, F.;Ducatelle, R.;Courtin,C.M.;Delcour,J.A.andBroekaert. W. F. (2008):Arabinoxylooligosaccharides from wheat bran inhibit Salmonella colonization in broiler chickens. Poult. Sci. 87:2329–2334.
  • Elsayed, N.A. (2002): Investigations on the significance of the gastrointestinal flora of the immune system of chickens. Ph.D thesis, Fac. of Vet.Med.,Leipziguniveristy.
  • Fature, A. A.andMatanmi, I. O. (2008): The effect of probiotics supplementation on the growth performance of two strains of cockerels, Journal of Central European Agriculture, 9, 405-410.
  • Fukata, T.; Sasai, K.; Miyamoto, T. and Baba, E. (1999): Inhibitory effects of competitive exclusion and fructooligosaccharide, singly and in combination, on Salmonella colonization of chicks. J. Food Protect. 62:229-233.
  • Gast, R.K. (2003): Paratyphoid infections. In: Diseases of Poultry eds. Y. M. Saif, H.J.Barones, J. R. Glisson, A. M. Fadly, L. R. McDougald, and D. E. Swayne. Pp.583-613. Ames, Iowa: BlackWell Publishing Co.
  • Gehad, A. Youssef.;Nashwa, A.; Ezzeldeen.; Ashgan, M.; Mostafa and Sherif, N.A. (2011): Effects of Isolated Lactobacillus acidophilus as a Probiotic on Chicken Vaccinated and Infected with Salmonella Typhimurium. Global Veterinaria 7 (5): 449-455, 2011.
  • Jarquin, R.L.; Nava, G.M.; Wolfenden, A.D.; Donoghue, A.M.; Hanning, I.; Higgins, S.E. and Hargis, B.M. 2007. The Evaluation of Organic Acids and Probiotic Cultures to Reduce Salmonella enteriditis Horizontal Transmission and Crop Infection in Broiler Chickens. Int. J. Poult. Sci., 6: 182-186. SAS Institute Inc., 2002. SAS user’s guide: statistics. SAS Institute Inc., Cary, N.C.
  • Hegazy, S. M.; and Y. Adachi. (2000). Comparison of the effects ofdietary selenium, zinc, and selenium and zinc supplementationongrowth and immune response between chick groups that wereinoculated with Salmonella and aflatoxin or Salmonella. Poult.Sci. 79:331–335.
  • Klose, V.; Mohnl, M.; Plail, R.; Schatzmayr, G. and Loibner, A.p. (2006): Development of acompetitive exclusion product meeting the regulatory requirements for registrationin the European Union, Mol. Nutr. Food Res.2006, 50, 563-571.
  • Král, M.; Angelovičová, M.; Mrázová, Ľ.; Tkáčová, J.; Kliment, M.( 2011): Probiotic and acetic acid of broilerchickens performance, Scientific Papers AnimalScience and Biotechnologies, 44, 149-152
  • Lister, S. A. (1988): Salmonella Enteritidis infection in broilers and broilerbreeders.Vet.Rec.,123,350.http://dx.doi.org/10.1136/vr.123.13.35
  • Lister, S. A.; and Barrow, P. (2008):Enterobacteriaceae. In Poultry Diseases (6th ed.) by Pattison, M., McMullin, P., Bradbury, j. and Alexander, D. Elsevier Ltd.
  • Marcq, C.; Cox, E.; Szalo, I.M.; Théwis, A.; and Beckers, Y. (2011): Salmonella Typhimurium oral challenge model in mature broilers: bacteriological immunological and growth performance aspects.Poultry Sci., 90: 59-67.
  • Marthedal, H.E. (1977): The occurrence of salmonellosis in poultry in Denmark 1935-1975, and the controlling programme established. In: Barnum D.A. (ed.) Proceedings of the international symposium on Salmonella and prospects for control. Guelph, Canada: University of Guelph, pp 78-94
  • Mayrhofer, S.; Paulsen, P.; Smulders, F. J. M. and Hilbert, F. (2003): Antimicrobial resistance profile of five major food-borne pathogens isolated from beef, pork and poultry. Inter. J. Food Microbiol., 97 (1), 23–29.http://dx.doi.org/10.1016/j.ijfoodmicro.2004.04.006.
  • Mountzouris,K.C.;Tsirtsikos,P.;Kalamara,E.;Nitsch,S.;Schatzmayr,G.;and Fegeros, K.(2007): Evaluation of the efficacy of a probiotic containing Lactobacillus,Bifidobacterium, Enterococcus, and Pediococcus strain in promoting broiler performance and modulating cecalmicroflora composition and metabolic activities. Poult.Sci. 86(2):309-317.
  • Mountzouris, K.C.; Tsirtsikos, P.; Palamidi, I.; Arvaniti, A.; Mohnl, M.; Schatzmayr, G.; and Fegeros, K. (2010): Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecalmicroflora composition. Poult. Sci., 89 (1): 58-67.
  • Murugkar, H.V.; Rahman, H. and Dutta, P. K. (2005): Distribution of virulence genes in Salmonella serovars isolated from man and animals. Indian Med. Res., 177, 66–70.
  • Padron, M. (1990): Salmonella Typhimurium outbreak in broiler chicken flocks in Mexico. Avian Dis 34, 221-223.
  • Pelicano, E.L.; Souza, P.A.; Souza, H.A.; Leonel, R.; Zeola, N.L. and Boiago, M.M (2004): Productive traits of broiler chickens fed diets containing different growth promoters. Brazilian Journal of Poultry Science, 6: 177-182.
  • Pervez, Rafiullah and Abdul Sajid (2011): Effect of feed additives on the performance of broilers Journal of Agricultural and Biological Science. 9: 66-71.
  • Rajput, I.R.; and LI, W.F. (2012): Potential role of probiotics in mechanism of intestinal immunity. Pak Vet J, 32: 303-308.
  • Saad, S.E.; Hamed, O.M.; Awaad, M.H. and Haveez, E. (1974): The possible role in chicken in the epidemiology of E.coli infection in infant.Vet.Cairo. Univeristy.25.481-486.
  • Schrezenmeir, J.; and Vrese, M. (2001): Probiotics, prebiotics and symbiotic approaching and definition. (Am. J. Clin. Nutr. 73(2): 361S-364S).
  • Sohail, M.U.; Ijaz, A. Yousaf, M.S.; Ashraf, K.; Zaneb,H.; Aleem, M. and Rehman, H. (2010): Alleviation of cyclic heat stress in broilers by dietary supplementation of mannan-oligosaccharide andLactobacillus-based probiotic: dynamics of cortisol,
  • Taheri, H.R.; Moravej, H.; Tabandeh, F.; Zaghari, M. and Shivazad,M. (2010): Efficacy of combined or single use of Lactobacillus crispatus LT116 and L.johnsonii LT171 on broiler performance. Br.Poult.Sci., 51 (5): 580-585.
  • Talebi, A.; Amirzadeh, B.; Mokhtari, B. and Gahri, H. (2008): Effects of amulti-strain probiotic (Primalac) on performance and antibody responses to Newcastle disease virus and infectious bursaldisease virus vaccination in broiler chickens. AvianPathol. 37 (5): 509-512.
  • Tellez, G.; Higgins, S. E.; Donoghue, A. M.; and Hargis, B. M. (2006): Digestive physiology and the role of microorganisms. J. Appl. Poult. Res. 15:136–144.
  • Vachkov, A.; Lyutskanov, M.; Petrov, V.; and Simeonov, R. (2004): Experimental E. coli infection in rabbits - clinical and morphological studies and attempts for control with an acidifyer. (BJVM, 7(3): 159-165).
  • Vandeplas, S.; Dubois Dauphin, R.; Thiry, C.; Beckers, Y.; Welling, G. W.; Thonart, P.; and Thewis. A. (2009): Efficiency of a Lactobacillusplantarum-xylanase combination on growth performances, microflora populations, and nutrient digestibilities of broilers infected with Salmonella Typhimurium. Poult. Sci. 88:1643–1654.
  • Yan, G.L.; Guo, Y.M.; and Yuan, J.M. (2011): Sodium alginate oligosaccharides from brown algae inhibit Salmonella Enteritidis colonization in broiler chickens. Poultry Science, v.90, p.1441-1448.
  • Zohair,G.A.M. (2006): "Recent prophylactic and control aspects of certain chicken bacterial problems".Ph.D.Vet.Med.Sci. (Diseases of birds and Rabbits) Fac.Vet.Med.Cairo Univ.