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Dataset Open Access

Expression and purification of PARP1 from Sf9 Insect cells 2019/02/04

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl

Project: Investigating HTT parylation and PAR-binding in its putative role in DNA damage repair

Experiment: Expression and purification of PARP1 from Sf9 Insect cells

Date completed:­ 2019/02/04

Rationale: HTT may be parylated or recognize PAR motifs on other proteins involved in DNA damage repair. To test this hypothesis, the full-length PARP1 cDNA was cloned in to pFBOH-SBP-TEV-LIC, overexpressed in insect cell Sf9 culture and purified. The purified PARP1 protein will be shared with Dr. Tamara Maiuri for her future experiments on the link between HTT protein and parylation. 

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
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