UV SPECTROPHOTOMETRIC ESTIMATION OF SUMATRIPTAN: STATISTICAL TREATMENT

Three simple, sensitive, selective and rapid UV spectrophotometric method was developed and validated for quantitative estimation of sumatriptan. The effect of three different solvents on quantification of sumatriptan in bulk and pharmaceutical formulations were studied. The standard solutions of sumatriptan were prepared with water and solvent blends containing Acetonitrile: water (1:1), Methanol: water (1:1). The standard solutions are scanned in the ultraviolet region ranges from 200nm-400nm. Sumatriptan showed λmax at 226nm in water, in case of Acetonitrile:water (1:1) the λmax was slightly shifted to 228nm and in Methanol: water (1:1) the λmax was found to be 226.5nm. Th method obeyed Beer-lamberts law over the concentration range of 2-18µg/ml. The method was validated by the parameters like Linearity, Accuracy, Precision, limit of Detection (LOD), Limit ofQuantification (LOQ), Molar Absorption Co-efficient, according to ICH guidelines. The validated parameters were treated statistically using ANOVA and significant differences were noticed. The study clearly indicated that the solvents having an influence on the estimation of sumatriptan succinate in bulk and formulations. The developed method was rapid and easy, so it can be applied for routine quality control analysis of sumatriptan from pharmaceutical dosage form.

Keywords: Sumatriptan Succinate, LOD, LOQ, ANOVA and UV Spectrophotometry.