Technical note Open Access

Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts

Joe Win; Emilie Chanclud; C. Sarai Reyes-Avila; Thorsten Langner; Tofazzal Islam; Sophien Kamoun

We report long-range sequencing of eight isolates of Magnaporthe oryzae(Syn. Pyricularia oryzae) from wheat, rice, foxtail millet and goosegrass using nanopore MinION. Our aim is to obtain chromosome-level genome assemblies that are freely available for public access to be scrutinized for genome rearrangements and structural variation.

Magnaporthe oryzae(Syn. Pyricularia oryzae) is a notorious fungus known for causing blast disease on rice and wheat with devastating effect on grain yield. M. oryzaehost range includes several other cereal crops such as oat, finger millet and foxtail millet as well as wild grasses. M. oryzaeis found all over the world wherever warm temperature and high humidity are common. Although lineages of M. oryzaetend to be adapted to a particular host, this pathogen can shift from one host to another when the conditions permit (Couch et al.2005). We hypothesize that structural variation contributes to adaptation to new hosts and environmental conditions following, for example, the model proposed by Chuma et al.(2011). To generate the genomics data that would enable such analyses, we sequenced eight isolates of M. oryzaefrom four different hosts using long-range nanonpore MinION with the aim of providing chromosome-level genome assemblies that are freely available for public access. 

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