FlexiDot – a customizable dotplot suite for sequence investigation and comparison

This is the presentation "FlexiDot – a customizable dotplot suite for sequence investigation and comparison", we gave in a meeting of TU Dresden's Plant Cell and Molecular Biology lab. With this talk we explain dotplots for molecular biology and present our FlexiDot dotplot tool.

First, we illustrate how a dotplot is calculated. Then, we introduce FlexiDot and compare it to other software. We explain how it works as well as its features. Subsequently, we exemplify the use of FlexiDot: Our showcases include tandem repeats (such as satellite and ribosomal DNA), transposable elements (such as LTR retrotransposons), and proteins with repeated domains (filamin proteins, nucleotide-binding leucine-rich repeat proteins).

More information on FlexiDot:

• Github: https://github.com/molbio-dresden/flexidot
• Publication: Seibt KM, Schmidt T, Heitkam T. 2018. FlexiDot: Highly customizable, ambiguity-aware dotplots for visual sequence analyses. Bioinformatics, 34(20): 3575–3577 - https://doi.org/10.1093/bioinformatics/bty395 - preprint version on github

If you use FlexiDot in your research, please cite our article.