Published December 1, 2018 | Version v1
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The EIMB Hydrogel Microarray Technology: Thirty Years Later

Creators

  • 1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Description

Biological microarrays (biochips) are analytical tools that can be used to implement complex integrative
genomic and proteomic approaches to the solution of problems of personalized medicine (e.g., patient
examination in order to reveal the disease long before the manifestation of clinical symptoms, assess the severity
of pathological or infectious processes, and choose a rational treatment). The efficiency of biochips is predicated
on their ability to perform multiple parallel specific reactions and to allow one to study the interactions of
biopolymer molecules, such as DNA, proteins, glycans, etc. One of the pioneers of microarray technology was the
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences (EIMB), with its suggestion to
immobilize molecular probes in the three-dimensional structure of a hydrophilic gel. Since the first experiments
on sequencing by hybridization on oligonucleotide microarrays conducted some 30 years ago, the hydrogel microarrays
designed at the EIMB have come a long and successful way from basic research to clinical laboratory
diagnostics. This review discusses the key aspects of hydrogel microarray technology and a number of state-ofthe-
art approaches for a multiplex analysis of DNA and the protein biomarkers of socially significant diseases,
including the molecular genetic, immunological, and epidemiological aspects of pathogenesis.

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