Journal article Open Access
C2C12 cells did not produce a large fold change in ALP expression following ligand administration (see previous post), therefore we will try to use FOP fibroblasts instead as these have a greater signalling response to BMP ligands. In order to complete this assay I must work out how many cells to seed in order to get a confluent well after 7 days of treatment (the expected period of time required for later compound assays). As the cells were very pale I employed a Calcein AM/Hoechst 3342 stain to mark viable cells.
190122_Optimisation of wild-type and FOP fibroblast seeding for ALP assay.pdf