Construction of Indonesian-Strain Avian Flu Virus Seed Vaccine Using Low Pathogenic Hemagglutinin Gene and Neuraminidase Pr8 Gene through Reverse Genetics

Avian Influenza H5N1 has been spreading in Indonesia since 2003. The Avian influenza virus H5N1 actually infects various animals, including birds, mammalian and others. Since 2005, avian influenza virus has infected humans. According to data, there are 132 persons suspected of contracting this virus, and this was one of the highest cases of avian influenza H5N1 outbreak in the world. One of the means of protection against infection is vaccination. Until now there is no H5N1 influenza vaccine extant, because the virus has a high level of pathogenicity, which makes it difficult to obtain a high yield of seed vaccine.