Dataset Open Access
Lasch, Peter;
Stämmler, Maren;
Schneider, Andy
(Version 20181130)
Version 3 (20181130) of the RKI’s MALDI-TOF mass spectral database represents the second update of the original database (version 20161027, https://doi.org/10.5281/zenodo.163517). The RKI database v.3 contains altogether 6264 mass spectra from highly pathogenic (i.e. biosafety level 3, BSL-3) bacteria such as Bacillus anthracis, Yersinia pestis, Burkholderia mallei, Burkholderia pseudomallei and Francisella tularensis as well as a selection of spectra from their close and more distant relatives. The database can be used as a reference for the diagnostics of BSL-3 bacteria using proprietary and free software packages for MALDI-TOF MS-based microbial identification. Spectral data are distributed as a 7-zip archive that contains the original mass spectra in its native data format (Bruker Daltonics). Please refer to the pdf file (181130-ZENODO-Metadata.pdf) to obtain information on cultivation condition, sample preparation and details of spectra acquisition. Do not try to print this document (~1000 pages!)
The pkf-file (181130_ZENODO_Peaklist_30Peaks_1.6.pkf) contains the MS peak list data in a Matlab compatible format. The latter data file can be imported into MicrobeMS, a Matlab-based free-of-charge software solution developed at RKI. MicrobeMS is available from http://www.microbe-ms.com.
The RKI mass spectral database will be updated on a regular basis.
The author's grateful thanks are given to the following persons for providing microbial strains and species, or mass spectra. Without their help this work would not be possible.
Name | Size | |
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181130-ZENODO-Metadata.pdf
md5:f6c22ea42d9b87cdee995cc7aa2ec2f6 |
3.5 MB | Download |
181130_ZENODO_Peaklist_30Peaks_1.6.pkf
md5:bcd41a3a2dbdd1b81ecdf32d402fd8ba |
10.7 MB | Download |
zenodo db 181130.7z
md5:963e5dd210353cb6b033a65878c7513e |
174.4 MB | Download |
Lasch P, Nattermann H, ... Grunow R, Naumann D, Anal Chem. (2008) 80(6): 2026-34.
Miñán A, Bosch A, Lasch P, ... , Naumann, D. Analyst. (2009) 134(6):1138-48.
Lasch P , ... , Naumann D. Appl Environ Microbiol. (2009) 75(22):7229-42.
Lasch P, ... , Naumann D. Anal Chem. (2010) 82(20):8464-75.
Lasch P & Naumann D. In: Proteomics, Glycomics and Antigenicity of BSL3 and BSL4 Agents. Wiley VCH 2011, Weinheim.
Wittwer M, Lasch P, .... , Jacob D, Grunow R. (2012) Applied Biosafety. 17(2): 59-63
Lasch P, Fleige C, Stämmler M, Layer F, Nübel U, Witte W, Werner G, J. (2014) Microbiol. Methods 100. 58-69.
Lasch P, ...., Stämmler M, Grunow R, Jacob D. (2015). J Clin Microbiol 53(8): 2632-2640.
Dieckmann R, ... , Dabrowski PW, Nitsche A, Stämmler M, ... , Lasch P. (2015), Faraday Discuss., DOI: 10.1039/C5FD00165J
Lasch P, Jacob D, Klee SR, Werner G, (2016). Applications of Mass Spectrometry in Microbiology, Springer International Publishing, pp. 203-234.
Lasch P, Grunow R, ...., Jacob D (2016). MALDI-TOF MS for the Identification of HPB. Trends in Analytical Chemistry
Lasch P, Jacob D, Grunow R, .., Doellinger J. (2016). Inactivation Techniques for MALDI-TOF MS. Trends in Analytical Chemistry
Lista, F., Reubsaet, F. A., ..., Paauw, A. (2011). Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS. BMC Microbiol 11(1): 267.
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