Reproducible in-silico omics analyses - Main figure

Figure 1: Nextflow produces stable analysis across different platforms. (a) Leishmania infantum clone JPCM5 genome annotation was predicted using a native and a dockerized (Debian Linux) version of the Companion eukaryotic annotation pipeline. The native and dockerized versions were run on Mac OSX and Amazon Linux platforms. The Venn diagram shows the existence of small, but significant discrepancies when comparing the genomic coordinates of predicted coding genes and non-coding RNAs, with some of these disparities including entire genes. (b) Results were deterministic on each platform, and totally identical readouts were measured when deploying the dockerized version. (c) A similar comparison carried out on a Kallisto/Sleuth pipeline when looking for differentially expressed genes (q-value <0.01) in an RNA-seq experiment collected from human lung fibroblasts reveals a comparable fluctuation between the Mac OSX and the Amazon Linux platform. Similarly, in this case both platforms produce identical readouts when deploying the dockerized version of the pipeline.