Cyclamen caulogenesis, rhizogenesis and microtuberization

The taxonomic status of Holostemma R.Br (Apocynaceae (formerly Asclepiadaceae), Asclepiadoideae) has recently (2014) been revised, allowing for biotechnology on this genus to assume a new and focused direction. This mini-review aims to cover what is known thus far about the tissue culture of Holostemma species, which includes medicinal species with ornamental potential. A post-publication peer review of the literature is provided, a critical assessment is made of the faults and weaknesses of the literature, and advice is offered as to how better to improve the research objectives to benefit researchers of Holostemma species.