SUB LETHAL EFFECTS OF METHOMYL PESTICIDE ON TISSUE-SPECIFIC ANTIOXIDATIVE RESPONSES IN A CHANNA STRIATUS

The aim of this study was to investigate the sub-lethal toxicity of a Methomyl-based insecticide effect of muscle, liver, gill, and kidney antioxidant enzyme activities of Channa striatus. Methomyl-based insecticides were treated sub-lethal concentration of 96 hours of methomyl exposure was 10% and 30% duration of 15 days and 30 days for murrel, Channa striatus. The muscle, liver, gill, and kidney antioxidant enzyme were analyzed. In muscles liver, gill and kidney tissue estimation of methomyl effects significantly (P>0.05) increased MDA, CAT and GST. MDA (Malondialdehyde), CAT (Catalase) and GST (Glutathione-s-transferase), of C.striatus increase in highest concentration of 0.162 for 30 days in different tissue when compared to control. The level of CAT (Catalase) in all groups and tissue not changed except (methomyl 40%). Estimation of all tissue GSH (Reduced glutathione) was significantly (P>0.05) decreased level of methomyl 40% insecticide-treated groups when compared to control. Simultaneously, MDA, CAT, and GST increased in muscle, liver, gill, and kidney tissue of 0.054ppm 15 days and 0.162ppm 30days, when compared to control, respectively. In the present study suggests that reactive oxygen species (ROS)-induced oxidative damage could be one of muscle, liver, gill and kidney toxic effect of methomyl 40% insecticide. The increase and alterations in the antioxidant defense system can be used as the pesticidecontaminated aquatic streams. Key words: sub-lethal concentration; MDA, CAT, GST, GSH, Channa striatus.