Presentation Open Access

An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila - sequence files

Li-Kroeger, David; Kanca, Oguz; Lee, Pei-Tseng; Cowan, Sierra; Lee, Michael T; Jaiswal, Manish; Salazar, Jose Luis; He, Yuchun; Zuo, Zhongyuan; Bellen, Hugo J.

In this work, Cas9 stocks for CRISPR experiments that carried an isogenic chromosome on either X, 2 or 3 derived from y w and were either y1 M{nos-Cas9.P}ZH-2A w* (from Bloomington Drosophila Stock Center) or y1 (iso X) w*; +/+; attP2(y+){nos-Cas9(y+)} (Ren et al., 2013) in which the y+ marker was mutagenized by injecting sgRNA expression plasmids (pCFD3-y1 and pCFD3-y2) against the yellow coding sequence.  These are the sequence files (.bam) and indexes (.bai) for the isogenic chromosomes (X, 2, 3) which were sequenced using whole genome sequencing (Human Genome Sequencing Center at Baylor College of Medicine).

Information on BAM files:

http://software.broadinstitute.org/software/igv/BAM

http://genome.ucsc.edu/goldenPath/help/bam.html

SAMtools download:

http://samtools.sourceforge.net/

Funding: Howard Hughes Medical Institute; National Institutes of Health U54HD083092, R01GM067858, T32NS043124; Robert A and Renee E Belfer Family Foundation
Files (22.7 GB)
Name Size
iso2_18.local_realigned.sorted.bam
md5:969a9d1528cf4206c2877ba1393c9e3e
8.3 GB Download
iso2_18.local_realigned.sorted.bam.bai
md5:67a93d7549c153d208a2f509db8722c3
458.1 kB Download
iso3_5.local_realigned.sorted.bam
md5:96e7cb14988acac55b9cf965f8ed16c9
6.2 GB Download
iso3_5.local_realigned.sorted.bam.bai
md5:37949f66f1c9ff37982acdc7d12d6794
454.2 kB Download
isoX_6.local_realigned.sorted.bam
md5:c28af7cb87700a09adc9e1ba3883d022
8.2 GB Download
isoX_6.local_realigned.sorted.bam.bai
md5:0933af13ace8a35ad701ba5e5e8901c9
457.3 kB Download
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