Dataset Open Access

miRNA gene regulatory networks for 38 human tissues

Kuijjer, Marieke Lydia; Quackenbush, John; Glass, Kimberly

We reconstructed miRNA regulatory networks for 38 tissues from the Genotype-Tissue Expression project (GTEx) using two different prior networks, one obtained with target predictions from TargetScan and one with target predictions from miRanda.

We used these networks to investigate gene expression and regulation by miRNAs across these tissues. In the RData file, we share the following objects:

- exp: a 16,161 by 9,435 data frame including normalized expression data for each sample.

- expTS: a 16,161 by 38 matrix including the tissue-specificity scores for each gene in each tissue.

- netT: a 10,391,523 by 41 data frame that includes the miRNA regulatory networks. The column "miRNA" includes the name of the regulating miRNA, the column "Gene" includes the target gene (HGNC symbol), and the column "Prior" the prior regulatory network based on target predictions from TargetScan, with 1 for edges that are canonical and 0 for edges that are non-canonical. The remaining 38 columns contain the PUMA network edge weights for each of the 38 tissues.

- netT_TS: a 10,391,523 by 38 matrix that includes the tissue-specificity scores of the miRNA regulatory networks that were modeled on the TargetScan prior. Edges are not labelled, but edge order corresponds to the edges in "netT".

- netM: a 10,391,523 by 41 data frame that includes the miRNA regulatory networks. The column "miRNA" includes the name of the regulating miRNA, the column "Gene" includes the target gene (HGNC symbol), and the column "Prior" the prior regulatory network based on target predictions from miRanda, with 1 for edges that are canonical and 0 for edges that are non-canonical. The remaining 38 columns contain the PUMA network edge weights for each of the 38 tissues.

- netM_TS: a 10,391,523 by 38 matrix that includes the tissue-specificity scores of the miRNA regulatory networks that were modeled on the miRanda prior. Edges are not labelled, but edge order corresponds to the edges in "netT".

- samples: a 9,435 by 2 data frame that includes sample identifiers (matching the identifiers in "exp") and the tissue to which these samples belong.

- mirnames: a 694 by 3 data frame that contains miRNA names of regulators and their matching target miRNA names. The first column "base_miRNA" contains the "base" miRNA, the name of the miRNA without any extensions. The second column "reg_miRNA" contains the 643 regulator miRNA, which may have -3P/-5P extensions, and which matches the miRNAs that are present as regulators in the networks. The third columns "tar_miRNA" contains the 621 target miRNAs, which may have numbered suffix extensions, and for which we have expression data available.

This work was supported by grants from the US National institutes of Health, including grants from the National Heart, Lung, and Blood Institute (5P01HL105339, 5R01HL111759, 5P01HL114501, K25HL133599), the National Cancer Institute (5P50CA127003, 1R35CA197449, 1U01CA190234, 5P30CA006516, P50CA165962), the National Institute of Allergy and Infectious Disease (5R01AI099204), and the Charles A. King Trust Postdoctoral Research Fellowship Program, Bank of America, N.A., Co-Trustees and Sara Elizabeth O'Brien Trust, Bank of America, N.A., Trustee. This work was conducted under dbGaP approved protocol #9112 (accession phs000424.v6.p1).
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