Effect of abscisic acid on depositing of the Karelian birch in vitro
Description
The possibility of prolonged continuous cultivation in vitro of explants of Karelian birch on media supplemented with abscisic acid (including those with a high content of sucrose, activated carbon, 6-BAP) is discussed in the paper.
Methods: culture in vitro; statistical analysis.
Periodic visual monitoring of the condition of micro-plants and (on occasion) transplanting plants to a fresh nutrient medium without hormones are necessary during depositing the Karelian birch in vitro. There is no negative effect of abscisic acid on the growth and development of birch explants sub-cultured on fresh media after different storage times (including cases with additional high contents of sucrose, activated carbon and 6-BAP to the nutrient medium).
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References
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