Published October 27, 2017 | Version v1
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A QUANTITATIVE AND QUALITATIVE EVALUATION OF HAEMATOPOIESIS IN THREE HABITAT SPECIALIST FISHES, ACANTHOCOBITIS BOTIA, DEVARIO AEQUIPINNATUS AND BARILIUS BARNA FROM A HILL STREAM ENVIRONMENT

Creators

  • 1. Aquatic Bioresource Research Laboratory, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata-700019, West Bengal, India

Description

The aim of the present study was to execute quantitative and qualitative assessments of developing blood cells in the head
kidney of three habitat specialist fish species of River Murti Acanthocobitis botia, Devario aequipinnatus and Barilius
barna. The variance in activity pattern and preference of specific habitat of these three fishes were correlated with their
haematopoietic profile. Eleven types of hematopoietic precursors and mature cells in erythroid and lymphoid lineage were
recognized in head kidneys of the three fish species. The erythroid lineage was found to be the most abundant as well as
diverse one with a special focus on the size and quantity of the small lymphoid haematoblasts, young and mature
erythrocytes. Significant difference regarding the rate of maturation of developing blood cells and erythropoietic efficiency
was found between these three species through cytological descriptions. These findings were corroborated through cell
cycle study by flow cytometry analysis, which showed high, medium and low values of S-phase cells in A. botia, B. barna
and D. aequipinnatus respectively, which corresponded to the turnover number that are associated with their respective
habitat. The differential pattern of erythropoiesis might indicate its functional significance in the employment of different
survival strategies that dictate varied activity and specialisation of habitat in fishes.

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Subjects

Zoology
10.5281/zenodo.1312080