SPEKcheck - fluorescence microscopy spectral visualisation and optimisation

SPEKcheck is a web application to visualise the efficiency of the light path in a fluorescence microscope. It can run locally and completely offline, or it can be configured to run in a site.

SPEKcheck models an optical setup as a detector, a dye, an excitation source, and an arbitrary number of filters in both the excitation and emission paths. SPEKcheck then reports values for the excitation efficiency of the dye, the collection efficiency of the emitted fluorescence, and a 'brightness' score, all easy comparison between different fluorescent labels. It also displays a spectral plot of various components, and the final emitted fluorescence.