Published June 12, 2018
| Version v0.2.4
Software
Open
hbc/bcbioRNASeq: bcbioRNASeq 0.2.4
- 1. Harvard (@hbc)
- 2. Harvard Chan School of Public Health
- 3. Harvard School of Public Health
Description
F1000v2 manuscript release version.
Major changesaggregateReplicates()
support has been added back. This function returns aRangedSummarizedExperiment
instead of abcbioRNASeq
object, containing only an aggregate raw counts matrix in thecounts
slot ofassays()
.- The functional analysis [R Markdown][] template has been reworked to use
dds_file
andorganism
as new parameter arguments. We've reduced the number of parameters required here to run [clusterProfiler][]. - Made
alphaSummary()
defunct forbcbioRNASeq
object, in favor ofDESeqDataSet
only. This function is only useful when a proper design formula has been defined.
metrics()
now contains an informative error for datasets that were analyzed using thefast-rnaseq
[bcbio][] pipeline.DESeqDataSet
coercion frombcbioRNASeq
object doesn't attempt to runDESeq()
command any more, which was unnecessary and improves speed.bcbioSingleCell()
constructor now supportscensorSamples
parameter. This is useful for removing known poor quality samples upon loading.- [ggplot2][] color and fill palettes are now set
NULL
in the quality control functions. This behavior doesn't change the appearance of the plot colors, which will still default toggplot2::scale_colour_hue()
orggplot2::scale_fill_hue()
. The upcoming [ggplot2][] v2.3.0 update supports global options for color and fill palettes, so these parameters may be deprecated in a future release. - Reworked the internal code for
topTables()
.
- Added [macOS][] testing to [Travis CI][] build checks.
- Fixed [clusterProfiler][] compilation error on [Travis CI][] by installing
libudunits2-dev
(Linux).
Files
hbc/bcbioRNASeq-v0.2.4.zip
Files
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Additional details
Related works
- Is supplement to
- https://github.com/hbc/bcbioRNASeq/tree/v0.2.4 (URL)