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Published June 12, 2018 | Version v0.2.4
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hbc/bcbioRNASeq: bcbioRNASeq 0.2.4

  • 1. Harvard (@hbc)
  • 2. Harvard Chan School of Public Health
  • 3. Harvard School of Public Health

Description

F1000v2 manuscript release version.

Major changes
  • aggregateReplicates() support has been added back. This function returns a RangedSummarizedExperiment instead of a bcbioRNASeq object, containing only an aggregate raw counts matrix in the counts slot of assays().
  • The functional analysis [R Markdown][] template has been reworked to use dds_file and organism as new parameter arguments. We've reduced the number of parameters required here to run [clusterProfiler][].
  • Made alphaSummary() defunct for bcbioRNASeq object, in favor of DESeqDataSet only. This function is only useful when a proper design formula has been defined.
Minor changes
  • metrics() now contains an informative error for datasets that were analyzed using the fast-rnaseq [bcbio][] pipeline.
  • DESeqDataSet coercion from bcbioRNASeq object doesn't attempt to run DESeq() command any more, which was unnecessary and improves speed.
  • bcbioSingleCell() constructor now supports censorSamples parameter. This is useful for removing known poor quality samples upon loading.
  • [ggplot2][] color and fill palettes are now set NULL in the quality control functions. This behavior doesn't change the appearance of the plot colors, which will still default to ggplot2::scale_colour_hue() or ggplot2::scale_fill_hue(). The upcoming [ggplot2][] v2.3.0 update supports global options for color and fill palettes, so these parameters may be deprecated in a future release.
  • Reworked the internal code for topTables().
Infrastructure changes
  • Added [macOS][] testing to [Travis CI][] build checks.
  • Fixed [clusterProfiler][] compilation error on [Travis CI][] by installing libudunits2-dev (Linux).

Files

hbc/bcbioRNASeq-v0.2.4.zip

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